The Cullens and the Swans

  • Kristen Stewart as Bella Swan, who finds herself surrounded by danger and targeted by the vengeful vampire Victoria. In the meantime, she must choose between her love for vampire Edward Cullen and her friendship with werewolf Jacob Black.
  • Robert Pattinson as Edward Cullen, Bella's vampire boyfriend who can read minds, except for Bella's.
  • Peter Facinelli as Carlisle Cullen, a compassionate doctor who leads the Cullen family.
  • Elizabeth Reaser as Esme Cullen, a loving mother figure of the Cullen clan.
  • Ashley Greene as Alice Cullen, a member of the Cullen family who can see "subjective" visions of the future and who is close friends with Bella.
  • Kellan Lutz as Emmett Cullen, the strongest member of the Cullen family.
  • Nikki Reed as Rosalie Hale, the most beautiful member of the Cullen family.
  • Jackson Rathbone as Jasper Hale, a member of the Cullen coven who trains his family on how to fight newborn vampires. The film also explores Jasper's backstory as a human during the American Civil War before he is turned into a vampire.
  • Billy Burke as Charlie Swan, Bella's father and Forks' Chief of Police.

Quileute tribe

  • Taylor Lautner as Jacob Black, a childhood friend of Bella's and a member of the Quileute tribe.
  • Chaske Spencer as Sam Uley, the leader of a werewolf pack that protects humans against predatory vampires.
  • Tinsel Korey as Emily Young, Sam's fiancée and a mother figure to the wolf pack.
  • Tyson Houseman as Quil Ateara, one of Jacob's best friends and the last to learn of his wolf heritage.
  • Alex Meraz as Paul, a volatile member of the wolf pack.
  • Kiowa Gordon as Embry Call, one of Jacob's best friends.
  • Bronson Pelletier as Jared, the joker of the wolf pack.
  • Julia Jones as Leah Clearwater.
  • Boo Boo Stewart as Seth Clearwater.
  • Gil Birmingham as Billy Black, Jacob's father, a friend of Bella's father, and a Quileute elder.

Nomadic vampires

  • Bryce Dallas Howard as Victoria, a vampire who wants to kill Bella to avenge her mate, James.
  • Xavier Samuel as Riley, a "good-looking college student who joins the plot of villain Victoria to murder protagonist Bella."
  • Jodelle Ferland as Bree, a newly turned vampire.
  • Catalina Sandino Moreno as Maria, the vampire who turned Jasper Hale.

The Volturi

  • Dakota Fanning as Jane, a guard of the Volturi who has the ability to torture people with illusions of pain.
  • Cameron Bright as Alec, Jane's brother.
  • Charlie Bewley as Demetri, a member of the Volturi who has "unparalleled tracking abilities".
  • Daniel Cudmore as Felix, a Volturi enforcer.

Humans

  • Anna Kendrick as Jessica Stanley, Bella's friend.
  • Justin Chon as Eric Yorkie, Bella's friend who dates Angela.
  • Christian Serratos as Angela Weber, Bella's friend.
  • Michael Welch as Mike Newton, Bella's friend who has a crush on her.
  • Jack Huston as Royce King II, "a human that lived during the Depression and was engaged to Rosalie."
  • Sarah Clarke as Renée Dwyer, Bella's mother